Specifying Gene Expression

Sci. Signal.  12 Feb 2008:
Vol. 1, Issue 6, pp. ec59
DOI: 10.1126/stke.16ec59

The efficiency of gene transcription by RNA polymerase II is modulated by the composition of promoter chromatin, including the presence of nucleosome-binding proteins. Using a genomic approach, Krishnakumar et al. show that poly(ADP-ribose) polymerase-1 (PARP-1) and the linker histone H1, two nucleosome-binding proteins that compete for binding to nucleosomes in vitro, exhibit a reciprocal pattern of binding at many promoters in vivo. At positively regulated promoters, PARP-1 is bound but H1 is not. Thus, promoter localization patterns of nucleosome-binding factors can specify global gene expression outcomes in vivo.

R. Krishnakumar, M. J. Gamble, K. M. Frizzell, J. G. Berrocal, M. Kininis, W. L. Kraus, Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes. Science 319, 819-821 (2008). [Abstract] [Full Text]