Research ArticleCancer

Multiepitope tissue analysis reveals SPPL3-mediated ADAM10 activation as a key step in the transformation of melanocytes

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Sci. Signal.  14 Mar 2017:
Vol. 10, Issue 470, eaai8288
DOI: 10.1126/scisignal.aai8288

Fig. S1. Screening algorithm to obtain melanoma-specific antibodies.

Fig. S2. BRAFV600E staining of melanoma tissue.

Fig. S3. Assessment of relative protein expression levels after MELC analysis.

Fig. S4. Melanoma- but not nevi-associated keratinocytes have an altered PEP, and an enhanced fluorescence signal reveals nuclear Notch1 staining.

Fig. S5. Tissue sections of different nevi and SSM growth phases analyzed by MELC.

Fig. S6. Quantification of the keratinocyte PEP in healthy skin, different nevi, and SSM and coimmunoprecipitation of ADAM10 with SPPL3.

Fig. S7. Expression of SPPL3, SPPL2a, and SPPL2b in keratinocytes associated with healthy skin, nevi, and SSM.

Fig. S8. Coimmunoprecipitation of the SPPL3 but not SPPL2a or SPPL2b with ADAM10 and subcellular localization of SPPL3 in melanoma cells.

Fig. S9. Abundance of PTEN in SSM, healthy skin, and a dysplastic nevus.

Fig. S10. Expression control of adenoviral constructs in primary melanocytes.

Fig. S11. BRAFV600E and PTEN modulate ADAM10 activity in melanoma cells.

Fig. S12. Hypothetical model on the activation and role of ADAM10 in melanocyte transformation.

Table S1. List of antibodies used for MELC analysis.

Table S2. Melanoma-associated keratinocytes change their PEP.