Research ArticleImmunology

Tetraspanin microdomains control localized protein kinase C signaling in B cells

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Sci. Signal.  09 May 2017:
Vol. 10, Issue 478, eaag2755
DOI: 10.1126/scisignal.aag2755

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Kinase recruiting by a tetraspanin

The protein kinase C (PKC) family member PKCβ mediates antigen-dependent B cell receptor (BCR) signaling to activate B cells. Mice deficient in the PKCβ isoform have defective antibody responses, whereas PKCβ-specific inhibitors are under investigation for the treatment of B cell malignancies. Using live-cell imaging of mouse and human B cells, Zuidscherwoude et al. showed that, in response to BCR stimulation, PKCβ was specifically and transiently recruited to plasma membrane microdomains enriched in the tetraspanin protein CD53. Mouse or human B cells deficient in CD53, but not other tetraspanins, showed impaired PKCβ activation and reduced phosphorylation of its targets, suggesting that CD53-containing membrane regions act as platforms for the activation of PKCβ in these cells.