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cAMPr: A single-wavelength fluorescent sensor for cyclic AMP

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Sci. Signal.  06 Mar 2018:
Vol. 11, Issue 520, eaah3738
DOI: 10.1126/scisignal.aah3738

Live-cell imaging of cAMP

The second messenger cAMP is found in many cell types and organisms. Increases in the cytosolic concentration and localization of cAMP mediate various cellular functions through the activation of the kinase PKA and the transcription factor CREB; thus, the ability to detect and image cAMP in live cells would help to understand its mechanism of action. Hackley et al. developed a genetically encoded fluorescent sensor of cAMP (cAMPr), which was expressed in mammalian neurons and in the Drosophila brain and responded to stimulation of adenylyl cyclase, the enzyme that generates cAMP. Furthermore, cAMPr was used together with a fluorescent Ca2+ sensor to simultaneously detect cAMP and Ca2+ in the Drosophila brain through two-photon imaging, suggesting its potential use to study cAMP signaling in diverse tissues and organisms.