Cell Adhesion Molecules Join Forces

Science's STKE  01 Dec 1999:
Vol. 1999, Issue 10, pp. tw2
DOI: 10.1126/stke.1999.10.tw2

When stimulated by specific antibodies, the leukocyte adhesion molecule DNAM-1 can trigger natural killer (NK) cell- or T cell-mediated cytotoxicity and cytokine production. Shibuya et al. report that this action may depend on DNAM-1 interaction with another leukocyte adhesion molecule, LFA-1. The two proteins were found in a complex in both NK cells and in activated T cells. In an NK cell line that lacked LFA-1, antibody-based ligation of DNAM-1 was unable to trigger cytolytic activity against target cells. Expression of LFA-1 in these NK cells restored DNAM-1-mediated cytotoxicity. In stimulated T cells, ligation of LFA-1 by either a specific antibody or its cognate ligand induced phosphorylation of DNAM-1 on tyrosine. The Src family kinase Fyn was also found in a complex with LFA-1 and DNAM-1. The authors propose that a signaling pathway in NK cells and T cells involves LFA-1 association with DNAM-1. It remains to be determined just how this interaction regulates cell adhesion or specific intracellular signaling events.

Shibuya, K., Lanier, L.L., Phillips, J.H., Ochs, H.D., Shimizu, K., Nakayama, E., Nakauchi, H., and Shibuya, A. (1999) Physical and functional association of LFA-1 with DNAM-1 adhesion molecule. Immunity 11: 615-623. [Online Journal]