Editors' ChoiceVirology

Microarrays Point to Mechanisms of Viral Transformation

Science's STKE  18 Apr 2000:
Vol. 2000, Issue 28, pp. tw11
DOI: 10.1126/stke.2000.28.tw11

Certain types of human papillomaviruses (HPVs) are associated with anogenital cancers. Chang and Laimins used microarray analysis to identify genes whose transcription is altered in response to latent HPV infection. Comparison of normal human keratinocytes (NHKs) and NHKs transfected with HPV DNA (HPV-31) showed that expression of 178 genes was increased in the HPV-31 cells, and 150 genes were repressed. The genes with increased expression did not display any obvious pattern, and many were unidentified expressed sequence tags (ESTs). The genes with decreased expression fell into three categories: (i) regulators of cell growth, (ii) keratinocyte-specific genes, and (iii) factors mediating the interferon response. The HPV-31 cells demonstrated a reduced response to interferon, indicating a functional correlation with the microarray results. Thus, microarray analysis of cells containing viral genomes points toward novel proteins involved in mediating the viral response and provides evidence for mechanisms of how virally infected cells can be prone to transformation.

Chang, Y.E. and Laimins, L.A. (2000) Microarray analysis identifies interferon-inducible genes and Stat-1 as major transcriptional targets of human papillomavirus type 31. J. Virol. 74: 4174-4182. [Abstract] [Full Text]