Editors' ChoiceMolecular Genetics

Homologous Recombination in Drosophila

Science's STKE  20 Jun 2000:
Vol. 2000, Issue 37, pp. tw4
DOI: 10.1126/stke.2000.37.tw4

T. H. Morgan's choice of Drosophila a century ago for studying the mechanisms of heredity has resulted in the lowly fruit fly's becoming one of the model organisms of biology. Drosophila has played a critical role in revolutions in genetics and molecular biology--the announcement in 1981 that the P transposable element could be used to create transgenic fruit flies also assured Drosophila a prominent place in genome research. Despite this utility and convenience, a couple of very important tools--homologous recombination and targeted mutagenesis--have been missing from the fruit fly molecular tool kit, tools that yeast and mouse researchers take for granted. Rong and Golic now report a method of gene targeting by homologous recombination in the fruit fly that may also find use in other organisms. A Perspective by Engels describes the method in greater detail.

Rong, Y.S., and Golic, K.G. (2000) Gene targeting by homologous recombination in Drosophila. Science 288: 2013-2018. [Abstract] [Full Text]

Engels, W.R. (2000) Genetics: Reversal of fortune for Drosophila geneticists? Science 288:1973-1975. [Full Text]