Cell Motility

Watching the Signals

Science's STKE  17 Oct 2000:
Vol. 2000, Issue 54, pp. tw2
DOI: 10.1126/stke.2000.54.tw2

How signaling molecules function is not only a matter of when but also a matter of where. Kraynov et al. demonstrate that activation of the small guanosine triphosphate-binding protein Rac1 can be visualized in real time in living cells. Activation of Rac1, known to induce actin-based morphological changes, was restricted to sites of actin polymerization, independent of overall intracellular Rac distribution. Thus, cells can produce distinct behaviors through specific distributions of activated Rac1.

Kraynov, V.S., Chamberlain, C., Bokoch, G.M., Schwartz, M.A., Slabaugh, S., and Hahn, K.M. (2000) Localized Rac activation dynamics visualized in living cells. Science 290: 333-337. [Full Text] [Abstract]