Protocol

Application of the Chromatin Immunoprecipitation Method to Identify in Vivo Protein-DNA Associations in Fission Yeast

Science's STKE  31 Oct 2000:
Vol. 2000, Issue 56, pp. pl1
DOI: 10.1126/stke.2000.56.pl1

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Abstract

The chromatin immunoprecipitation (ChIP) method provides an ideal tool for detecting direct or indirect interactions between proteins of interest and DNAs with known sequences. Here, we introduce the ChIP protocol used in our laboratory to identify in vivo protein-DNA association in the fission yeast Schizosaccharomyces pombe. The cytological and genetic merits of the fission yeast for studying control of the eukaryotic cell cycle and chromosome dynamics are reinforced by application of this ChIP method.

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