Improved Cell Culture

Free-Ranging Fibroblasts

Science's STKE  27 Nov 2001:
Vol. 2001, Issue 110, pp. tw434
DOI: 10.1126/stke.2001.110.tw434

The fibroblast has been the prototype cell for studying a range of phenomena, including cell proliferation, morphology, adhesion, and movement. Yet, our current understanding of fibroblast biology is based predominantly on observing these cells in two-dimensional (2D) culture. Cukierman et al. (see the Perspective by Geiger) have designed a 3D matrix system to culture fibroblasts and report key biological differences compared with the traditional 2D culture system. A comprehensive description of cell-matrix adhesions reveals striking structural and morphological differences that may justify a reevaluation of current models.

E. Cukierman, R. Pankov, D. R. Stevens, K. M. Yamada, Taking cell-matrix adhesions to the third dimension. Science 294, 1708-1712 (2001). [Abstract] [Full Text]

B. Geiger, Encounters in space. Science 294, 1661-1663 (2001). [Full Text]