Editors' ChoiceFunctional Genomics

Clues from Close and Distant Relatives

Science's STKE  08 Jan 2002:
Vol. 2002, Issue 114, pp. tw12
DOI: 10.1126/stke.2002.114.tw12

The DNA damage response (DDR) process in eukaryotes includes DNA damage-induced checkpoint control pathways and DNA repair processes. Boulton et al. describes a combination of protein-protein interaction mapping and high-throughput RNA-mediated interference to identify 23 genes in Caenorhabditis elegans that are required for DDR, 11 of which had not been implicated previously. One of the genes identified is the ortholog of human BCL3, a gene frequently altered in chronic lymphocytic leukemia. A first-generation physical map of the chimpanzee genome has been generated that will provide a solid basis and resources for analysis of its genome. Fujiyama et al. have mapped more than 60,000 bacterial artificial chromosomes (BACs) from a chimpanzee genome library to the human genome by means of BAC-end sequences to search for relatively large rearrangements between both genomes. Two clusters containing large, nonrandom differences were found relative to human chromosome 21.

S. J. Boulton, A. Gartner, J. Reboul, P. Vaglio, N. Dyson, D. E. Hill, M. Vidal, Combined functional genomic maps of the C. elegans DNA damage response. Science 295, 127-131 (2002). [Abstract] [Full Text]

A. Fujiyama, H. Watanabe, A. Toyoda, T. D. Taylor, T. Itoh, S.-F. Tsai, H.-S. Park, M.-L. Yaspo, H. Lehrach, Z. Chen, G. Fu, N. Saitou, K. Osoegawa, P. J. de Jong, Y. Suto, M. Hattori, Y. Sakaki, Construction and analysis of a human-chimpanzee comparative clone map. Science 295, 131-134 (2002). [Abstract] [Full Text]