Protocol

Flow Cytometric Analysis of T Cell Receptor Signal Transduction

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Science's STKE  16 Apr 2002:
Vol. 2002, Issue 128, pp. pl5
DOI: 10.1126/stke.2002.128.pl5

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Abstract

Intracellular assays of signal transduction in T cells stimulated by antigen in vivo have been limited by requirements for large numbers of highly purified cells to perform conventional biochemical techniques. Here, we describe a technique using flow cytometry to study signaling events in T cells after antigen recognition in the body.

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