Persistently Interfering

Science's STKE  23 Apr 2002:
Vol. 2002, Issue 129, pp. tw150
DOI: 10.1126/stke.2002.129.tw150

RNA interference (RNAi), in which short, double-stranded RNAs of 21 to 25 nucleotides result in the destruction of messenger (and other) RNAs bearing homologous sequences, has become a powerful tool in the experimental manipulation of gene expression. Results have already demonstrated the efficacy of transiently knocking down or knocking out gene expression in mammalian cells using RNAi. Brummelkamp et al. have now developed a vector system that can direct the persistent synthesis of small, interfering RNAs in mammalian cells and can efficiently suppress gene expression for months.

T. R. Brummelkamp, R. Bernards, R. Agami, A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550-553 (2002). [Abstract] [Full Text]