Working with the Mediator

Science's STKE  30 Apr 2002:
Vol. 2002, Issue 130, pp. tw161
DOI: 10.1126/stke.2002.130.tw161

The mechanisms involved in transcriptional activation of genes transcribed by the eukaryotic RNA polymerase II are not yet fully understood. Transcriptional activators recruit coactivators to a promoter, which in turn associate with and regulate the polymerase molecule. In yeast, the Mediator coactivator complex is recruited to promoters through interactions between transcriptional activator proteins and specific subunits of the Mediator. Stevens et al. extend this model to higher eukaryotics by demonstrating that specific activators can mediate transcriptional responses to the Ras-mitogen-activated protein kinase signal transduction pathway through specific interactions with the Sur2 subunit of the mammalian Mediator complex.

J. L. Stevens, G. T. Cantin, G. Wang, A. Shevchenko, A. Shevchenko, A. J. Berk, Transcription control by E1A and MAP kinase pathway via Sur2 Mediator subunit. Science 296, 755-758 (2002). [Abstract] [Full Text]