Enumeration, Phenotyping, and Identification of Activation Events in Conjugates Between T cells and Antigen-Presenting Cells by Flow Cytometry

Science's STKE  10 Sep 2002:
Vol. 2002, Issue 149, pp. pl14
DOI: 10.1126/stke.2002.149.pl14

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Microscopic analysis of T cell-antigen-presenting cell (T cell:APC) interactions at the single cell level has been a powerful, but tedious and subjective, technique. In this paper, we describe a rapid and quantitative method to identify T cell:APC conjugates using succinimidyl ester dyes, which irreversibly label free amine groups on the cell surface. The labeled cell conjugates and subsequent activation events are detected by flow cytometry.

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