Editors' ChoiceGENETIC SCREENS

Positive Interference

STKE  01 Apr 2003:
Vol. 2003, Issue 176, pp. tw130-TW130
DOI: 10.1126/stke.2003.176.tw130

RNA interference (RNAi) is now often used to block gene expression and to create a phenotype that, it is hoped, gives clues into the gene's function. Lum et al. have developed a high-throughput genome-scale screen that uses RNAi and cultured Drosophila cells to identify new components of a specific signaling pathway rapidly and systematically. The method identified several new positive and negative regulators of the Hedgehog signaling pathway, some of which were only associated with the Wingless signaling pathway. This RNAi-based approach to functional genomics may allow a more complete view of signaling mechanisms and regulatory crosstalk between pathways.

L. Lum, S. Yao, B. Mozer, A. Rovescalli, D. Von Kessler, M. Nirenberg, P. A. Beachy, Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells. Science 299, 2039-2045 (2003). [Abstract] [Full Text]