High-Resolution Multicolor Imaging of Dynamic Signaling Complexes in T Cells Stimulated by Planar Substrates

See allHide authors and affiliations

Science's STKE  08 Apr 2003:
Vol. 2003, Issue 177, pp. pl8
DOI: 10.1126/stke.2003.177.pl8

You are currently viewing the abstract.

View Full Text


The dynamic visualization of developing immunological synapses has been hindered by the difficulty of imaging the contact between the T cell and the antigen-presenting cell (APC). Here, we describe a technique in which T cell responses are constrained to a planar stimulatory substrate. This approach, when used in conjunction with immunofluorescent staining procedures or fluorescent protein tags, greatly facilitates detection of the dynamic molecular rearrangements that accompany the formation of contacts and the initiation of signal transduction through the T cell receptor (TCR). Using this method, we have observed signaling complexes of dynamically varying compositions that possess distinct fates.

View Full Text