Editors' ChoicePlant biology

Understanding Ethylene

Science's STKE  05 Aug 2003:
Vol. 2003, Issue 194, pp. tw308-TW308
DOI: 10.1126/stke.2003.194.tw308

The development of tools that help in the functional characterization of genes is important as genomic advances continue. Alonso et al. developed a genome-wide collection of sequence-indexed insertion mutants in the model plant Arabidopsis thaliana. Nearly 2/3 of the total complement of Arabidopsis genes were individually knocked out by generating over 225,000 insertional mutations with Agrobacterium transferred DNA. Integration did not occur randomly at the chromosome or gene level. The authors have used this resource to identify new genes that are involved in responses to the plant hormone ethylene.

J. M. Alonso, A. N. Stepanova, T. J. Leisse, C. J. Kim, H. Chen, P. Shinn, D. K. Stevenson, J. Zimmerman, P. Barajas, R. Cheuk, C. Gadrinab, C. Heller, A. Jeske, E. Koesema, C. C. Meyers, H. Parker, L. Prednis, Y. Ansari, N. Choy, H. Deen, M. Geralt, N. Hazari, E. Hom, M. Karnes, C. Mulholland, R. Ndubaku, I. Schmidt, P. Guzman, L. Aguilar-Henonin, M. Schmid, D. Weigel, D. E. Carter, T. Marchand, E. Risseeuw, D. Brogden, A. Zeko, W. L. Crosby, C. C. Berry, J. R. Ecker, Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301, 653-657 (2003). [Abstract] [Full Text]