RNA Binding Proteins and the Control of Motor Activity

Science's STKE  18 Nov 2003:
Vol. 2003, Issue 209, pp. tw452-TW452
DOI: 10.1126/stke.2092003TW452

RNA binding proteins can play important roles in gene regulation and are also associated with a large number of human diseases. Identifying the sets of RNAs to which these proteins bind is critical to understanding their normal functions and roles in disease. Nova is a tissue-specific splicing factor involved in the disease paraneoplastic opsoclonus myoclonus ataxia (POMA), in which patients cannot inhibit movement and suffer uncontrollable shaking. Ule et al. developed an ultraviolet cross-linking and immunoprecipitation method to purify Nova-RNA complexes. Nova regulates a set of RNA targets that code for proteins involved in neuronal inhibition or that function at the synapse, consistent with POMA's being a disorder of motor inhibition.

J. Ule, K. B. Jensen, M. Ruggiu, A. Mele, A. Ule, R. B. Darnell, CLIP identifies Nova-regulated RNA networks in the brain. Science 302, 1212-1215 (2003). [Abstract] [Full Text]