Stimulation of Cells Using EGF-Coated Magnetic Beads

Science's STKE  03 Feb 2004:
Vol. 2004, Issue 218, pp. pl1
DOI: 10.1126/stke.2182004pl1

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The ability to determine the spatial localization of signals can assist in defining the regulation of specific signaling pathways within the cell. Localized application of a stimulus, followed by imaging of various proteins or their phosphorylated products, allows visualization of the propagation scales of responses to the stimulus. To obtain a localized stimulus of epidermal growth factor (EGF), we bound the ligand to magnetic beads, which could be rapidly pulled onto cell surfaces by a magnet. This method allows the spatial analysis of EGF-induced signaling pathways.

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