Genome-Wide Functional Analysis

Science's STKE  10 Feb 2004:
Vol. 2004, Issue 219, pp. tw56
DOI: 10.1126/stke.2192004TW56

An RNA interference-based screen on a genome-wide scale is likely to become one of the methods of choice for comprehensive functional genomic analysis because of its potential to identify, rapidly and systematically, genes that control a variety of cellular processes. Boutros et al. conducted such a screen of the Drosophila genome in tissue culture cells and identified new genes and orthologs of human disease genes that control cell growth and survival. The results provide new functional information for annotating the genome of this important model organism.

M. Boutros, A. A. Kiger, S. Armknecht, K. Kerr, M. Hild, B. Koch, S. A. Haas, Heidelberg Fly Array Consortium, R. Paro, N. Perrimon, Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303, 832-835 (2004). [Abstract] [Full Text]