Imaging Calcium Concentration Dynamics in Small Neuronal Compartments

Sci. STKE, 10 February 2004
Vol. 2004, Issue 219, p. pl5
DOI: 10.1126/stke.2192004pl5

Imaging Calcium Concentration Dynamics in Small Neuronal Compartments

  1. Ryohei Yasuda1,
  2. Esther A. Nimchinsky1,3,
  3. Volker Scheuss1,
  4. Thomas A. Pologruto1,2,
  5. Thomas G. Oertner1,4,
  6. Bernardo L. Sabatini1,5, and
  7. Karel Svoboda1,*
  1. 1Howard Hughes Medical Institute, The Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
  2. 2Graduate Program in Biophysics, Harvard University, Cambridge, MA 02138, USA.
  3. 3Present address: Center for Molecular and Behavioral Neuroscience Aidekman Research Center, Rutgers State University, Newark Campus, 197 University Avenue, Newark, NJ 07102, USA.
  4. 4Present address: Friedrich Miescher Institute, WRO-1066.4.10, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
  5. 5Present address: Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
  1. *Corresponding author. Telephone, 516-367-6878; fax, 516-367-8866; e-mail, svoboda{at}cshl.edu

Abstract

Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.

Citation:

R. Yasuda, E. A. Nimchinsky, V. Scheuss, T. A. Pologruto, T. G. Oertner, B. L. Sabatini, and K. Svoboda, Imaging Calcium Concentration Dynamics in Small Neuronal Compartments. Sci. STKE 2004, pl5 (2004).

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