Editors' ChoiceVESICLE FUSION

Sensing Calcium During Membrane Fusion

Science's STKE  20 Apr 2004:
Vol. 2004, Issue 229, pp. tw147-TW147
DOI: 10.1126/stke.2292004TW147

The minimal machinery required for calcium-stimulated fusion, such as occurs during regulated secretion or neurotransmitter release, has been the subject of much controversy. Using a reconstituted system, Tucker et al. now show a robust requirement for the cytoplasmic tail of the synaptic vesicle protein synaptotagmin to act as a calcium sensor during SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor)-mediated fusion in reconstituted liposomes.

W. C. Tucker, T. Weber, E. R. Chapman, Reconstitution of Ca2+-regulated membrane fusion by synaptotagmin and SNAREs. Science 304, 435-438 (2004). [Abstract] [Full Text]

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