Illuminating Drug Discovery

Science's STKE  16 Nov 2004:
Vol. 2004, Issue 259, pp. tw416
DOI: 10.1126/stke.2592004tw416

In drug discovery, profiling technologies are important for measuring drug actions both on the desired target and on other targets. High-throughput methods such as transcriptional and proteomic profiling provide data on the responses of populations of cells, normally at single-drug concentrations. Perlman et al. describe a method for high-throughput cytological profiling that combines automated microscopy, image analysis, and statistical analysis. Using multiple fluorescent probes that cover a range of cell biological functions, they profiled the dose-dependent effects of 100 drugs on cultured mammalian cells. They succeeded in categorizing drugs of known mechanism and suggested targets for drugs of uncertain mechanism.

Z. E. Perlman, M. D. Slack, Y. Feng, T. J. Mitchison, L. F. Wu, S. J. Altschuler, Multidimensional drug profiling by automated microscopy. Science 306, 1194-1198 (2004). [Abstract] [Full Text]