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Fixing Broken DNA

Science's STKE  21 Dec 2004:
Vol. 2004, Issue 264, pp. tw460
DOI: 10.1126/stke.2642004tw460

Repair of DNA double-strand breaks is critical for genome stability, and cells have developed mechanisms to repair these chromosomal defects. It has been proposed that modified histones at double-strand breaks may attract chromatin-remodeling complexes that allow access for the DNA repair machinery. Kusch et al. characterize a chromatin-remodeling complex involved in histone exchange during genome repair. The protein complex from Drosophila includes proteins such as chromatin remodeling enzymes and histone variants. The complex facilitates DNA repair by acetylating the phosphorylated histone and then exchanging the modified protein with an unmodified version to create an altered chromatin structure at the DNA lesion.

T. Kusch, L. Florens, W. H. MacDonald, S. K. Swanson, R. L. Glaser, J. R. Yates, III, S. M. Abmayr, M. P. Washburn, J. L. Workman, Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions. Science 306, 2084-2087 (2004). [Abstract] [Full Text]