Interfering with RNA Interference

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Science's STKE  24 May 2005:
Vol. 2005, Issue 285, pp. tw201
DOI: 10.1126/stke.2852005tw201

RNA interference (RNAi) is central to a number of natural RNA-based silencing processes and is becoming a common tool used in a wide range of studies in eukaryotes. It is also being explored for its therapeutic potential. Kim et al. carried out a genome-wide screen in Caenorhabditis elegans for components of the RNAi pathway using RNAi. Although apparently a "circular" methodology, the screen identified 90 viable and lethal genes involved in RNAi, most of which were not previously linked with the process. Classes of factors include RNA binding and processing factors, chromatin-associated factors, and nuclear import and export factors. The screen also provides insight into the degree of overlap between different RNAi-based silencing pathways.

J. K. Kim, H. W. Gabel, R. S. Kamath, M. Tewari, A. Pasquinelli, J.-F. Rual, S. Kennedy, M. Dybbs, N. Bertin, J. M. Kaplan, M. Vidal, G. Ruvkun, Functional genomic analysis of RNA interference in C. elegans. Science 308, 1164-1167 (2005). [Abstract] [Full Text]