Editors' ChoiceCell Biology

Enzyme Kinetics in Living Color

Science's STKE  09 Jan 2007:
Vol. 2007, Issue 368, pp. tw17
DOI: 10.1126/stke.3682007tw17

Innovative methods are required to study the spatial regulation of enzymatic activity inside living cells. Yudushkin et al. describe such a method based on the detection of enzyme-substrate complexes using fluorescence lifetime imaging microscopy. The detection of the enzyme interacting with the substrate ensures utmost specificity and enables evaluation of the localized activity of a particular enzymatic species. The technique was used to investigate the spatial regulation of growth factor signaling by the tyrosine phosphatase PTP1B and revealed that PTP1B exists inside cells as kinetically distinct, spatially separated subpopulations.

I. A. Yudushkin, A. Schleifenbaum, A. Kinkhabwala, B. G. Neel, C. Schultz, P. I. H. Bastiaens, Live-cell imaging of enzyme-substrate interaction reveals spatial regulation of PTP1B. Science 315, 115-119 (2007). [Abstract] [Full Text]