Editors' ChoiceGENETICS

Finding Redox-Active Cysteines

Science's STKE  23 Jan 2007:
Vol. 2007, Issue 370, pp. tw33
DOI: 10.1126/stke.3702007tw33

Cysteine residues are found in a wide variety of proteins, in which they serve many functions. Identifying particular functions has required laborious individual characterization. Fomenko et al. present and experimentally verify an algorithm that can identify which cysteines act in redox reactions by searching for sporadic selenocysteine-Cys pairs in sequence databases. This methodology should assist in metagenomic analyses to identify oxidoreductase families.

D. E. Fomenko, W. Xing, B. M. Adair, D. J. Thomas, V. N. Gladyshev, High-throughput identification of catalytic redox-active cysteine residues. Science 315, 387-389 (2007). [Abstract] [Full Text]