Differential Responses to Change

Science's STKE  01 May 2007:
Vol. 2007, Issue 384, pp. tw146
DOI: 10.1126/stke.3842007tw146

Understanding how organisms respond to genetic or environmental perturbations requires quantitative measurements across multiple levels of biological information. Ishii et al. used a variety of technologies to measure gene expression, protein levels, metabolite concentrations, and reaction fluxes to study the response of Escherichia coli to growth rates and genetic deletions. Although E. coli responds to changes in the environment by regulating the level of enzyme expression to keep metabolite levels stable, messenger RNA and protein levels do not change in response to most gene disruptants. Instead, there are likely changes in the structure of the metabolic network that keep the levels of most metabolites stable.

N. Ishii, K. Nakahigashi, T. Baba, M. Robert, T. Soga, A. Kanai, T. Hirasawa, M. Naba, K. Hirai, A. Hoque, P. Y. Ho, Y. Kakazu, K. Sugawara, S. Igarashi, S. Harada, T. Masuda, N. Sugiyama, T. Togashi, M. Hasegawa, Y. Takai, K. Yugi, K. Arakawa, N. Iwata, Y. Toya, Y. Nakayama, T. Nishioka, K. Shimizu, H. Mori, M. Tomita, Multiple high-throughput analyses monitor the response of E. coli to perturbations. Science 316, 593-597 (2007). [Abstract] [Full Text]

U. Sauer, M. Heinemann, N. Zamboni, Getting closer to the whole picture. Science 316, 550-551 (2007). [Summary] [Full Text]