Teaching ResourceBiochemistry

A Biochemical Assay for Acetylcholinesterase Activity in PC12 Cells

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Science's STKE  10 Jul 2007:
Vol. 2007, Issue 394, pp. tr2
DOI: 10.1126/stke.3942007tr2

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This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data. The lab is intended to be used in conjunction with a cell culture lab in which PC12 cells are exposed to various agents that influence their phenotypic state.

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