A Biochemical Assay for Acetylcholinesterase Activity in PC12 Cells

Sci. STKE, 10 July 2007
Vol. 2007, Issue 394, p. tr2
DOI: 10.1126/stke.3942007tr2

A Biochemical Assay for Acetylcholinesterase Activity in PC12 Cells

  1. Paul J. Schwartz1,
  2. Jay A. Blundon2, and
  3. Elizabeth M. Adler3,*
  1. 1Department of Neurological Surgery, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA.
  2. 2Department of Biology, Rhodes College, Memphis, TN 38112, USA.
  3. 3Associate Editor of Science’s STKE, American Association for the Advancement of Science, 1200 New York Avenue, NW, Washington, DC 20005, USA.
  1. *Corresponding author. E-mail, eadler{at}aaas.org

Abstract

This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data. The lab is intended to be used in conjunction with a cell culture lab in which PC12 cells are exposed to various agents that influence their phenotypic state.

Citation:

P. J. Schwartz, J. A. Blundon, and E. M. Adler, A Biochemical Assay for Acetylcholinesterase Activity in PC12 Cells. Sci. STKE 2007, tr2 (2007).
Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882