Teaching ResourceBiochemistry

Introduction to Enzyme Kinetics: Assay of β-Galactosidase

Science's STKE  10 Jul 2007:
Vol. 2007, Issue 394, pp. tr4
DOI: 10.1126/stke.3942006tr4

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Abstract

This tutorial describes a colorometric enzyme assay to determine simple enzyme kinetics. It is designed as an introduction for beginning laboratory students in a biology or biochemistry course, and requires no previous understanding of chemistry. It may be used as an assignment to prepare students for a laboratory exercise in enzyme kinetics Through this tutorial, students will gain an understanding of the procedures for measuring enzyme activity, using the specific example of an assay for the bacterial enzyme, β-galactosidase.

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