Research ArticleCell Migration

Antagonistic Regulation of Actin Dynamics and Cell Motility by TRPC5 and TRPC6 Channels

Science Signaling  26 Oct 2010:
Vol. 3, Issue 145, pp. ra77
DOI: 10.1126/scisignal.2001200

You are currently viewing the abstract.

View Full Text
As a service to the community, AAAS/Science has made this article free with registration.

This article has a correction. Please see:


The Rho family of small guanosine triphosphatases (Rho GTPases: RhoA, Cdc42, and Rac1) regulates many aspects of cell behavior, including actin dynamics and cell migration. The generation of calcium ion (Ca2+) microdomains is critical in promoting cell migration because they control the localized activity of Rho GTPases. We identified receptor-activated TRPC5 and TRPC6 (transient receptor potential canonical type 5 and 6) channels as antagonistic regulators of actin remodeling and cell motility in fibroblasts and kidney podocytes. We show that TRPC5 is in a molecular complex with Rac1, whereas TRPC6 is in a molecular complex with RhoA. TRPC5-mediated Ca2+ influx induces Rac1 activation, thereby promoting cell migration, whereas TRPC6-mediated Ca2+ influx increases RhoA activity, thereby inhibiting cell migration. Our data unveil antagonistic Ca2+ influx pathways as a conserved signaling mechanism for the integrated regulation of cell migration.

View Full Text

Cited By...