Sorting Out the Centrosome

Science Signaling  28 Jun 2011:
Vol. 4, Issue 179, pp. ec179
DOI: 10.1126/scisignal.4179ec179

The centrosome is the paired organelle that organizes microtubules to form the mitotic spindle. The yeast centrosome (called the spindle pole body) is composed of 18 proteins. To help better understand how the centrosome is regulated, Keck et al. made a comprehensive analysis of phosphorylation of the yeast centrosome proteins by mass spectroscopy. Almost 300 sites of phosphorylation were identified, about 100 of which occurred only during mitosis. The results may help point the way for further functional characterization of the more complicated human centrosome, which, with some 100 proteins, is likely to be regulated by a very large set of phosphorylation events.

J. M. Keck, M. H. Jones, C. C. L. Wong, J. Binkley, D. Chen, S. L. Jaspersen, E. P. Holinger, T. Xu, M. Niepel, M. P. Rout, J. Vogel, A. Sidow, J. R. Yates III, M. Winey, A cell cycle phosphoproteome of the yeast centrosome. Science 332, 1557–1561 (2011). [Abstract] [Full Text]