Structural Biology

Dissecting DNA Repair

Sci. Signal.  15 May 2012:
Vol. 5, Issue 224, pp. ec140
DOI: 10.1126/scisignal.2003215

Covalent modification of proteins can be a crucial regulatory event. Poly(ADP-ribose) polymerase 1 (PARP-1) synthesizes poly(ADP-ribose), which is attached to and regulates proteins involved in DNA repair. Langelier et al. (see the Perspective by Gagné et al.) use x-ray crystallography and biochemical analysis to demonstrate how PARP-1 detects DNA damage and how the interaction with DNA is coupled to poly(ADP-ribosyl)ation activity.

M.-F. Langelier, J. L. Planck, S. Roy, J. M. Pascal, Structural basis for DNA damage–dependent poly(ADP-ribosyl)ation by human PARP-1. Science 336, 728–732 (2012). [Abstract] [Full Text]

J.-P. Gagné, M. Rouleau, G. G. Poirier, PARP-1 activation—Bringing the pieces together. Science 336, 678–679 (2012). [Summary] [Full Text]