An Interaction Between BZR1 and DELLAs Mediates Direct Signaling Crosstalk Between Brassinosteroids and Gibberellins in Arabidopsis

Sci. Signal., 2 October 2012
Vol. 5, Issue 244, p. ra72
DOI: 10.1126/scisignal.2002908

An Interaction Between BZR1 and DELLAs Mediates Direct Signaling Crosstalk Between Brassinosteroids and Gibberellins in Arabidopsis

  1. Qian-Feng Li,
  2. Chunming Wang,
  3. Lei Jiang,
  4. Shuo Li,
  5. Samuel S. M. Sun*, and
  6. Jun-Xian He*
  1. State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, China.
  1. *To whom correspondence should be addressed. E-mail: jxhe{at}cuhk.edu.hk (J.-X.H.); ssun{at}cuhk.edu.hk (S.S.M.S.)

Abstract

Plant growth and development are coordinated by several groups of small-molecule hormones, including brassinosteroids (BRs) and gibberellins (GAs). Physiological and molecular studies have suggested the existence of crosstalk between BR and GA signaling. We report that BZR1, a key transcription factor activated by BR signaling, interacts in vitro and in vivo with REPRESSOR OF ga1-3 (RGA), a member of the DELLA family of transcriptional regulators that inhibits the GA signaling pathway in Arabidopsis thaliana. Genetic analyses of plants with mutations in the genes encoding RGA and BZR1 revealed that RGA suppressed root and hypocotyl elongation of the gain-of-function mutant bzr1-1D. Ectopic expression of proteins of the DELLA family reduced the abundance and transcriptional activity of BZR1. Reporter gene analyses further indicated that BZR1 and RGA antagonize each other’s transcriptional activity. Our data indicated that BZR1 and RGA served as positive and negative regulators, respectively, of both the BR and the GA signaling pathways and establish DELLAs as mediators of signaling crosstalk between BRs and GAs in controlling cell elongation and regulation of plant growth.

Citation:

Q.-F. Li, C. Wang, L. Jiang, S. Li, S. S. Sun, and J.-X. He, An Interaction Between BZR1 and DELLAs Mediates Direct Signaling Crosstalk Between Brassinosteroids and Gibberellins in Arabidopsis. Sci. Signal. 5, ra72 (2012).

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