Protein Complexes and Target Genes Identification by in Vivo Biotinylation: The STAT5 Paradigm

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Sci. Signal.  30 Oct 2012:
Vol. 5, Issue 248, pp. pt13
DOI: 10.1126/scisignal.2003622

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A Presentation from the European Society for Paediatric Endocrinology (ESPE) New Inroads to Child Health (NICHe) Conference on Stress Response and Child Health in Heraklion, Crete, Greece, 18 to 20 May 2012.


Signal transducers and activators of transcription (STATs) are implicated in physiological cellular processes, stress-related responses, and malignancies. This Presentation provides an overview on STATs, focusing on STAT5, and describes an in vivo biotinylation methodology applied in mammalian cells for identification of STAT5-containing protein complexes and STAT5 target genes. Our results show that in vivo biotinylation can be used efficiently to elucidate the complex STAT5-dependent transcriptional mechanisms.

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