Structural Biology

G Structures

Science Signaling  07 Jan 2014:
Vol. 7, Issue 307, pp. ec4
DOI: 10.1126/scisignal.2005041

G protein–coupled receptors (GPCRs) are eukaryotic membrane proteins that have a central role in cellular communication and have become key drug targets. To overcome the difficulties of growing GPCR crystals, Liu et al. used an x-ray free-electron laser to determine a high-resolution structure of the serotonin receptor from microcrystals.

W. Liu, D. Wacker, C. Gati, G. W. Han, D. James, D. Wang, G. Nelson, U. Weierstall, V. Katritch, A. Barty, N. A. Zatsepin, D. Li, M. Messerschmidt, S. Boutet, G. J. Williams, J. E. Koglin, M. M. Seibert, C. Wang, S. T. A. Shah, S. Basu, R. Fromme, C. Kupitz, K. N. Rendek, I. Grotjohann, P. Fromme, R. A. Kirian, K. R. Beyerlein, T. A. White, H. N. Chapman, M. Caffrey, J. C. H. Spence, R. C. Stevens, V. Cherezov, Serial femtosecond crystallography of G protein–coupled receptors. Science 342, 1521–1524 (2013). [Abstract] [Full Text]