Editors' ChoiceNeuroscience

Causing Chloride Changes

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Sci. Signal.  11 Feb 2014:
Vol. 7, Issue 312, pp. ec41
DOI: 10.1126/scisignal.2005162

Because intracellular chloride concentrations largely determine the direction and magnitude of current flow through GABAA channels, the stability of intracellular chloride concentration is important to maintain consistent synaptic inhibition. Glykys et al. examined the mechanisms by which chloride gradients in neurons are established, using chloride imaging with transgenically expressed Clomeleon dye. Surprisingly, intracellular chloride was not primarily determined by transporters. Instead, subcellular gradients of immobile anions generated inverse chloride gradients.

J. Glykys, V. Dzhala, K. Egawa, T Balena, Y. Saponjian, K. V. Kuchibhotla, B. J. Bacskai, K. T. Kahle, T. Zeuthen, K. J. Staley, Local impermeant anions establish the neuronal chloride concentration. Science 343, 670–675 (2014). [Abstract] [Full Text]