Research ResourcePosttranslational modification

Proteome-Wide Identification of SUMO2 Modification Sites

Sci. Signal.  29 Apr 2014:
Vol. 7, Issue 323, pp. rs2
DOI: 10.1126/scisignal.2005146

You are currently viewing the editor's summary.

View Full Text

Sleuthing SUMO Sites

Covalent attachment to small ubiquitin-like modifiers (SUMOs) alters the stability, localization, and function of diverse proteins. SUMO-specific enzymes transfer SUMO to lysines on target proteins in a process known as sumoylation. Tammsalu et al. present a method to discover the exact sites to which SUMOs conjugate. Overexpression of a mutated form of SUMO2 and subsequent cleavage with a sequence-specific endoproteinase resulted in tagged sumoylated peptides. Antibody-based enrichment of tagged peptides and analyses by proteomics identified more than 1000 sumoylated sites in the human proteome, thus providing a wealth of information for future studies.