Movie 2

Sci. STKE, 22 October 2002
DOI: 10.1126/stke.2002.155.pe43

Movie 2. Screening for state transition mutants.

Leaves of the thale cress (Arabidopsis thaliana) were imaged by a camera that detects chlorophyll fluorescence. Fluorescence is initially high (seen as the red color), and falls after 6 min in the light (the movie is 10 times normal speed), as LHC II becomes phosphorylated and the chloroplasts undergo the transition to state 2. A far-red is then switched on, and fluorescence falls further, to rise slightly after a further 2 min, as the phosphate group is removed from phospho-LHC II, resulting in the transition to state 1. One plant, whose two leaves are seen near the center, behaves differently, and its fluorescence stays high.

Citation: J. F. Allen, H. L. Race, Will the real LHC II kinase please step forward? Science's STKE (2002), http://www.stke.org/cgi/content/full/sigtrans;2002/155/pe43.


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Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882