Erratum

Erratum

The following corrections should be noted for the Protocol "Monitoring Apoptosis with Fluorescent Zn2+-Indicators" by Kimura et al.

Recipe 3 was missing the directions for the preparation of the Stock Solution. This Stock Solution should also be used in Recipes 7 and 8.

Recipe 3: Zn2+-Detection Solutions
Stock Solution
Add 3.2 mg of L1•(HCl)4•(H2O)2 to 0.5 ml of HBSS for a final concentration of 10 mM Store at 4°C.
Zn2+-Detection Solution 1
Add 50 µl of Stock Solution to 5 ml of HBSS for a final concentration of 100 µM. Store at 4°C.


Recipe 7: Apoptosis-Detection Solution 1
ReagentAmountFinal Concentration
L1•(HCl)4•(H2O)2 Stock Solution50 µl100 µM
(Recipe 3)  
PI, 10 mM15 µl30 µM
Add to 5 ml of HBSS. Store at 4°C.


Recipe 8: Apoptosis-Detection Solution 2
ReagentAmountFinal Concentration
L1•(HCl)4•(H2O)2 Stock Solution50 µl100 µM
(Recipe 3)  
PI1.0 mg30 µM
Annexin V-Cy350 µl1:100
Prepare in 5 ml of the binding buffer included in the Annexin V-Cy3 kit. Use immediately.

A corrected PDF is available starting April 27, 2004.

A PDF of the protocol as originally published with the incorrect recipes is also available http://stke.sciencemag.org/cgi/data/sigtrans;2004/223/pl7/DC1/1.