Supplementary Materials

Supplementary Materials for
Cripto Localizes Nodal at the Limiting Membrane of Early Endosomes

Marie-Hélène Blanchet,1 J. Ann Le Good,1 Viola Oorschot,2 Stéphane Baflast,1 Gabriella Minchiotti,3 Judith Klumperman,2 Daniel B. Constam1*

1Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland.

2Cell Microscopy Center and Department of Cell Biology, University Medical Centre Utrecht, Heidelberglaan 100, 3584CX Utrecht, the Netherlands.

3Institute of Genetics and Biophysics "A. Buzzati-Traverso," Via P. Castellino 111, 80131 Naples, Italy.

This PDF file includes:

  • Fig. S1. Activin and TGF-β receptor signaling in COS1 cells.
  • Fig. S2. Monitoring of Nodal endocytosis by antibody-uptake experiments.
  • Fig. S3. Nodal is not internalized in fluid-phase carriers marked with fluorescent dextran.
  • Fig. S4. Localization of Cripto and Nodal in early endosomes marked by EEA1.
  • Fig. S5. Sorting of internalized biotin-EGF to the lumen of early endosomes is not inhibited by Cripto.

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*To whom correspondence should be addressed. E-mail: Daniel.Constam{at}

Citation: M.-H. Blanchet, J. A. Le Good, V. Oorschot, S. Baflast, G. Minchiotti, J. Klumperman, D. B. Constam, Cripto localizes Nodal at the Limiting Membrane of Early Endosomes. Sci. Signal.1, ra13 (2008).

© 2008 American Association for the Advancement of Science