Characterization of the Intrinsic and TSC2-GAP�Regulated GTPase Activity of Rheb by Real-Time NMR
Christopher B. Marshall, Jason Ho,1 Claudia Buerger, Michael J. Plevin, Guang-Yao Li, Zhihong Li, Mitsuhiko Ikura,* Vuk Stambolic*
This PDF file includes:
- Fig. S1. Role of Rheb in mTOR signaling.
- Fig. S2. Spectra of Rheb-GDP and Rheb-GMPPNP.
- Fig. S3. Secondary structure and chemical shift index analysis.
- Fig. S4. Preparation of Rheb-GTP for GTPase assay.
- Fig. S5. Time course of GTP hydrolysis of Rheb-GTP.
- Fig. S6. Analysis of Rheb-GTP and Rheb-GDP during GTP hydrolysis.
- Fig. S7. Circular dichroism spectra of WT TSC21225�1742 and its variants.
- Fig. S8. Further development of real-time NMR method to increase temporal
- Fig. S9. Further development of real-time NMR method to decrease sample requirement.
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1Division of Signaling Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M9. 2Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2M9.
*To whom correspondence should be addressed. E-mail,(V.S.) and (M.I.)