Supplementary Materials

Sci. Signal., 24 February 2009
DOI: 10.1126/scisignal.2000113
Supplementary Materials for:

Dok-7 Activates the Muscle Receptor Kinase MuSK and Shapes Synapse Formation

Akane Inoue, Kiyoko Setoguchi, Yosuke Matsubara, Kumiko Okada, Nozomi Sato, Yoichiro Iwakura, Osamu Higuchi, Yuji Yamanashi*

*To whom correspondence should be addressed. E-mail: yyamanas{at}

This PDF file includes:

  • Fig. S1. The pHSA-Dok-7-EGFP transgene is overexpressed in skeletal muscle.
  • Fig. S2. The number of myofibers was comparable in WT and Dok-7 Tg mice.
  • Fig. S3. AChR clusters formed only in Dok-7–/– myotubes that express Dok-7-EGFP.
  • Fig. S4. Transcripts for endogenous Dok-7 were distributed uniformly throughout the muscle at E18.5.
  • Fig. S5. The Myc epitope appropriately increased the molecular mass of phosphorylated MuSK-cyt.
  • Fig. S6. Dok-7 activated MuSK-cyt.
  • Fig. S7. MuSK was not activated by a Dok-7 deletion mutant lacking the PH domain or the Dok-family protein Dok-1.
  • Fig. S8. The pHSA-Dok-7-Myc transgene is overexpressed in skeletal muscle.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2,981 KB

Citation: A. Inoue, K. Setoguchi, Y. Matsubara, K. Okada, N. Sato, Y. Iwakura, O. Higuchi, Y. Yamanashi, Dok-7 activates the muscle receptor kinase MuSK and shapes synapse formation. Sci. Signal.2, ra7 (2009).

© 2009 American Association for the Advancement of Science

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882