Supplementary Materials

Supplementary Materials for:

Dynamic Signaling in the Hog1 MAPK Pathway Relies on High Basal Signal Transduction

Javier Macia, Sergi Regot, Tom Peeters, N�ria Conde, Ricard Solé,* Francesc Posas*

*To whom correspondence should be addressed. E-mail: ricard.sole{at} (R.S.) and francesc.posas{at} (F.P.)

This PDF file includes:

  • Mathematical Analysis
  • Fig. S1. Schematic diagram of the HOG pathway.
  • Fig. S2. Pbs2 activity is a limiting factor to determine amplitude of Hog1 phosphorylation in response to osmotic stress.
  • Fig. S3. Inhibition of Hog1 results in phosphorylation of the MAPK.
  • Fig. S4. Inhibited Hog1 remains mainly cytoplasmic.
  • Fig. S5. Inhibition of transcription or translation does not prevent phosphorylation of Hog1.
  • Fig. S6. Deletion of FPS1 does not abolish hog1as phosphorylation in the presence of inhibitor.
  • Fig. S7. Neither Sho1 nor Ste50 is essential for phosphorylation of inactive Hog1.
  • Fig. S8. The Sln1 branch is essential for rapid response to osmotic stress.
  • Fig. S9. The kinases Fus3 and Kss1 receive basal signaling in the absence of stimuli and differently control a negative feedback loop.

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Citation: J. Macia, S. Regot, T. Peeters, N. Conde, R. Solé, F. Posas, Dynamic Signaling in the Hog1 MAPK Pathway Relies on High Basal Signal Transduction. Sci. Signal.2, ra13 (2009).

© 2009 American Association for the Advancement of Science