Supplementary Materials

Supplementary Materials for:

Spatiotemporal Patterning During T Cell Activation Is Highly Diverse

Kentner L. Singleton, Kole T. Roybal, Yi Sun, Guo Fu, Nicholas R. J. Gascoigne, Nicolai S. C. van Oers, Christoph Wülfing*

*To whom correspondence should be addressed. E-mail: christoph.wuelfing{at}

This PDF file includes:

  • Supplementary Text
  • Methods
  • Fig. S1. Components of T cell activation examined in this study.
  • Fig. S2. FACS-based sorting of sensor-expressing T cells.
  • Fig. S3. Quantification of the abundance of biosensors and endogenous PKCθ.
  • Fig. S4. Rapid nuclear translocation of NFAT.
  • Fig. S5. Patterning of PKCθ in activated 5C.C7 T cells.
  • Fig. S6. Highly diverse spatiotemporal patterning during T cell activation.
  • Fig. S7. Dynamic nature of spatiotemporal patterns.
  • Fig. S8. Requirement for TCRζ ITAMs for effective recruitment of TCR to the cSMAC signaling complex.
  • Fig. S9. Enrichment of sensors in the large T cell invagination.
  • Fig. S10. Requirement for Rho activity for the resolution of the large T cell invagination.
  • Fig. S11. High diversity of spatiotemporal patterning during T cell activation.
  • Fig. S12. Variations in spatiotemporal patterning in activated T cells from different TCR transgenic mice.
  • Fig. S13. Reduced TCR clustering in TH2-polarized DO11.10 T cells.
  • Fig. S14. Regulation of spatiotemporal patterning by costimulation.
  • Fig. S15. Delayed central accumulation of the TCR compared to that of its dependent signaling intermediates.
  • Fig. S16. Similar dynamics of translocation of tubulin-GFP and the Arf6 sensor from the uropod to the interface upon formation of the T cell�APC interface.
  • Table S1. List of supplementary movies and a key that includes the sensor monitored, the time of cell coupling, and a description of the critical features observed.
  • Table S2. Numbers of cell couples analyzed.
  • References

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Other Supplementary Material for this manuscript includes the following:

Movies S1 to S27 (MOV format)

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Citation: K. L. Singleton, K. T. Roybal, Y. Sun, G. Fu, N. R. J. Gascoigne, N. S. C. van Oers, C. Wülfing, Spatiotemporal patterning during T cell activation is highly diverse. Sci. Signal.2, ra15 (2009).

© 2009 American Association for the Advancement of Science