Supplementary Materials

Supplementary Materials for:

Therapeutically Targeting ErbB3: A Key Node in Ligand-Induced Activation of the ErbB Receptor–PI3K Axis

Birgit Schoeberl, Emily A. Pace, Jonathan B. Fitzgerald, Brian D. Harms, Lihui Xu, Lin Nie, Bryan Linggi, Ashish Kalra, Violette Paragas, Raghida Bukhalid, Viara Grantcharova, Neeraj Kohli, Kip A. West, Magdalena Leszczyniecka, Michael J. Feldhaus, Arthur J. Kudla, Ulrik B. Nielsen*

*To whom correspondence should be addressed. E-mail: unielsen{at}merrimackpharma.com

This PDF file includes:

  • Supplementary Text: Model Development
  • Fig. S1. Characterization of MM-121 in OvCar8 and DU145 cell lines.
  • Table S1. Measured ErbB receptor expression and mutation status for investigated cell lines.
  • Table S2A. Characterization of ErbB1 phosphorylation dose-response curves for ErbB1 binding ligands and HRG1-β.
  • Table S2B. Characterization of ErbB2 phosphorylation dose-response curves for ErbB1 binding ligands and HRG1-β.
  • Table S2C. Characterization of ErbB3 phosphorylation dose-response curves for ErbB1 binding ligands and HRG1-β.
  • Table S2D. Characterization of AKT phosphorylation dose-response curves for ErbB1 binding ligands and HRG1-β.
  • Table S3. Initial amounts of nonzero species in the computational model.
  • Table S4. Summary of biochemical reactions implemented into the computational model using mass action kinetics with corresponding parameters.
  • Table S5. Description of parameters with values. Parameter number corresponds to the first reaction in which that parameter appears.
  • Table S6. Sensitive parameters controlling ErbB3, ErbB2, ErbB1, and AKT phosphorylation.
  • Table S7. Summary of biochemical reactions describing the implementation of the inhibitors: MM-121, cetuximab, pertuzumab, and lapatinib.
  • Table S8. Inhibitor parameter values.
  • References

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Citation: B. Schoeberl, E. A. Pace, J. B. Fitzgerald, B. D. Harms, L. Xu, L. Nie, B. Linggi, A. Kalra, V. Paragas, R. Bukhalid, V. Grantcharova, N. Kohli, K. A. West, M. Leszczyniecka, M. J. Feldhaus, A. J. Kudla, U. B. Nielsen, Therapeutically targeting ErbB3: A key node in ligand-induced activation of the ErbB receptor�PI3K axis. Sci. Signal.2, ra31 (2009).

© 2009 American Association for the Advancement of Science