Supplementary Materials

Supplementary Materials for:

The Plant NADPH Oxidase RBOHD Mediates Rapid Systemic Signaling in Response to Diverse Stimuli

Gad Miller, Karen Schlauch, Rachel Tam, Diego Cortes, Miguel A. Torres, Vladimir Shulaev, Jeffery L. Dangl, Ron Mittler*

*To whom correspondence should be addressed. E-mail: ronm{at}

This PDF file includes:

  • Fig. S1. A model of the ROS-mediated autopropagating long-distance rapid signal in plants.
  • Fig. S2. qRT-PCR analysis of the rapid systemic expression of Zat12, ankyrin repeat family protein, WRKY18, WRKY40, and WRKY53 in wild type and mutants deficient in RBOHD (rbohD), RBOHF (rbohF), or RBOHC (rbohC).
  • Fig. S3. Detection of the rapid systemic signal in response to local application of high light intensity, cold, heat, or salinity stresses.
  • Table S1. Pharmacological treatments used to study the rapid systemic signal with the experimental design shown in Fig. 3.
  • Table S2. Transcripts down-regulated in systemic leaves of Arabidopsis plants 10 min after wounding.
  • Descriptions of Movies S1 to S5.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1. Suppression of the rapid wound-induced systemic signal in Zat12::Luc and Zat12::Luc/rbohD rosette leaves.
  • Movie S2. Rapid wound-induced systemic signal in inflorescences of control Zat12::Luc and Zat12::Luc/rbohD plants.
  • Movie S3. Local wound-induced accumulation of ROS in wild-type and rbohD plants.
  • Movie S4. Delay in the progression of the rapid systemic signal by application of DPI at distal location.
  • Movie S5. Delay in the progression of the rapid systemic signal by application of catalase at distal location.

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Citation: G. Miller, K. Schlauch, R. Tam, D. Cortes, M. A. Torres, V. Shulaev, J. L. Dangl, R. Mittler, The plant NADPH oxidase RBOHD mediates rapid systemic signaling in response to diverse stimuli. Sci. Signal. 2, ra45 (2009).

© 2009 American Association for the Advancement of Science