Quantitative Phosphoproteomic Analysis of T Cell Receptor Signaling Reveals System-Wide Modulation of Protein-Protein Interactions
Viveka Mayya, Deborah H. Lundgren, Sun-Il Hwang, Karim Rezaul, Linfeng Wu, Jimmy K. Eng, Vladimir Rodionov, David K. Han*
*To whom correspondence should be addressed. E-mail:
This PDF file includes:
- Materials and Methods (incorporating figs. S1 to S4)
- Fig. S5. Accumulation of unique phosphopeptides in the Jurkat phosphoproteome.
- Fig. S6. Distribution of the number of sites in phosphopeptides and the certainty in their localization.
- Fig. S7. Summary of TCR-responsive fold changes in the abundance of phosphopeptides as determined by SILAC experiments.
- Fig. S8. Illustration of the quantification of fold change by targeted MS/MS.
- Fig. S9. Comparison of phosphopeptide spectral count data with SILAC data.
- Fig. S10. Global trends in the phosphorylation data set.
- Fig. S11. Abundance and subcellular localization of phosphoproteins based on protein spectral count data.
- Fig. S12. Putative substrates of ERK during TCR signaling.
- Fig. S13. Validation of Thr260 of BCL11B as a target of ERK.
- Fig. S14. Predicted substrates of Zap70.
- Fig. S15. Primary sequence representation of transcriptional regulators with TCR-responsive phosphorylation sites.
- Fig. S16. Interactions among proteins involved in alternative splicing of mRNA that have TCR-responsive phosphorylation sites.
- Fig. S17. TCR-responsive phosphorylation events in nuclear pore components.
- Fig. S18. Network analysis to assess the influence of inducible phosphorylation of Ser or Thr residues on PPIs.
- Fig. S19. Typical product ion chromatogram showing the lack of phosphorylation in polymerized microtubules.
- Fig. S20. Network representation of nuclear proteins involved in hematopoiesis, lymphoma, and leukemia for which phosphorylation sites were identified in the current study.
- Fig. S21. Schematic of the experimental work flow used to identify TCR-responsive phosphorylation sites by SILAC.
- Fig. S22. Phosphorylated forms of tubulins are excluded from polymerized microtubules.
- Descriptions of supplementary tables
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Other Supplementary Material for this manuscript includes the following:
Tables S1 to S14
Format: Microsoft Excel format (XLS) (Zipped)
Size: 5.0 MB